EPR and NMR study of molecular components mobility and organization in goat milk under ultrasound treatment

Ultrasonics Sonochemistry

Irina Barashkova¹  Nor Saadah M. Yusof²  Mikhail Motyakin³  Victoria Zaborova³  Andrey Sergeev³  Olga Krasulya⁴ 

[1] Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow 119334 Russia;Moscow Institute of Physics and Technology (National Research University), Moscow Region 141700 Russia;N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, Moscow 119991 Russia;Sechenov First Moscow State Medical University, Moscow 119991 Russia;

关键词: Ultrasound;    Goat milk;    EPR spin probe;    Spin trap;    NMR relaxation;    Diffusion;   

DOI  :  

来源: DOAJ

【 摘 要 】

The effect of ultrasound treatment on molecular mobility and organization of the main components in raw goat milk was studied by EPR and NMR spectroscopies. NMR relaxation studies showed an increase in the spin–lattice T1 and spin–spin T2 relaxation times in goat milk products (cream, anhydrous fat) and change in the diffusion of proton-containing molecules during ultrasound treatment. The diffusion became more uniform and could be rather accurately approximated by one effective diffusion coefficient Deff, which indicates homogenization of goat milk components, dispersion of globular and supermicellar formations under sonication. EPR studies have shown that molecular mobility and organization of hydrophobic regions in goat milk are similar to those observed in micellar formations of surfactants with a hydrocarbon chain length C12–C16. Ultrasound treatment did not affect submicellar and protein globule organization. Free radicals arising under ultrasound impact of milk reacted quickly with components of goat milk (triglycerides, proteins, fatty acids) and were not observed by spin trapping method.

【 授权许可】

Unknown