In vitro gas production kinetics and degradability of a diet for growing lambs: effect of fibrolytic enzyme products at different dose levels

Italian Journal of Animal Science

Fernando Sánchez-Dávila¹  Javier Hernández-Meléndez²  Santiago Joaquin²  Salomón Ruíz²  Abdel-Fattah Z. M. Salem³  José Fernando Vázquez-Armijo⁴  Rolando Rojo⁴  Nicolás López-Villalobos⁴  Daniel López-Aguirre⁴ 

[1] Autonomous University of Nuevo Leon;Autonomous University of Tamaulipas;Autonomous University of the State of Mexico;Centro Universitario, Autonomous University of the State of Mexico;

关键词: degradability;    exogenous enzymes;    gas production;    kinetics;    lambs;   

DOI  :  10.1080/1828051X.2016.1209088

来源: DOAJ

【 摘 要 】

The objective of this study was to evaluate the effects of three fibrolytic enzyme products (cellulase (CEL), xylanase (XYL) and a 1:1 mixture of CEL and XYL (MIX)) at three dose levels (0, 1 and 3 μL/0.5 g DM) on the in vitro fermentation of a diet for growing lambs. Bottles were incubated for 96 h at 39 °C. A mathematical model was used to estimate the parameters describing the gas production (GP) curve (b, c and L). Dry matter degradability (DMD) and fibre (NDFD and ADFD) degradability were determined at the end of the incubation period. Metabolisable energy (ME) and short chain fatty acids (SCFA) were calculated at 24 h of incubation. The asymptotic GP (parameter b) was affected (p < 0.02) by enzyme product and dose level, with a significant linear response (p < 0.05). Dose level affected ME and SCFA with a significant linear (p < 0.05) and quadratic (p < 0.01) response. The interaction between enzyme product and dose level was significant (p < 0.05) for cumulative GP up to 72 and 96 h of incubation, pH, ADFD and DMD. The results suggest that application of exogenous cellulases has the potential to alter asymptotic GP and degradability of ADF and DM of a diet for growing lambs, but most of the results depend on the interaction between enzyme product and dose level. Future studies are required to determine the ideal combination between enzyme product and dose level for optimal degradation of ruminant feeds.

【 授权许可】

Unknown