Nitric-oxide-driven oxygen release in anoxic Pseudomonas aeruginosa

iScience

Peter Østrup Jensen¹  Mads Lichtenberg²  Tim Tolker-Nielsen²  Thomas Bjarnsholt²  Laura Line²  Tim Holm Jakobsen²  Morten Levin Rybtke²  Mette Kolpen³  Verena Schrameyer⁴  Michael Kühl⁴  Masanori Toyofuku⁵  Nobuhiko Nomura⁵ 

[1] Department of Clinical Microbiology, Rigshospitalet, 2100 Copenhagen, Denmark;Costerton Biofilm Center, Department of Immunology and Microbiology, Faculty of Health Sciences, University of Copenhagen, 2200 Copenhagen, Denmark;Department of Clinical Microbiology, Rigshospitalet, 2100 Copenhagen, Denmark;Marine Biological Section, Department of Biology, University of Copenhagen, 3000 Helsingør, Denmark;Microbiology Research Center for Sustainability (MiCS), Faculty of Life and Environmental Sciences, University of Tsukuba, 305-8577 Tsukuba, Japan;

关键词: Biological sciences;    Microbiology;    Microbial physiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Denitrification supports anoxic growth of Pseudomonas aeruginosa in infections. Moreover, denitrification may provide oxygen (O2) resulting from dismutation of the denitrification intermediate nitric oxide (NO) as seen in Methylomirabilis oxyfera. To examine the prevalence of NO dismutation we studied O2 release by P. aeruginosa in airtight vials. P. aeruginosa rapidly depleted O2 but NO supplementation generated peaks of O2 at the onset of anoxia, and we demonstrate a direct role of NO in the O2 release. However, we were not able to detect genetic evidence for putative NO dismutases.The supply of endogenous O2 at the onset of anoxia could play an adaptive role when P. aeruginosa enters anaerobiosis. Furthermore, O2 generation by NO dismutation may be more widespread than indicated by the reports on the distribution of homologues genes. In general, NO dismutation may allow removal of nitrate by denitrification without release of the very potent greenhouse gas, nitrous oxide.

【 授权许可】

Unknown