Low-Temperature Storage Improves the Over-Time Stability of Implantable Glucose and Lactate Biosensors

Sensors

Andrea Bacciu¹  Rossana Migheli¹  Giulia Puggioni¹  Silvia Fancello¹  Gaia Rocchitta¹  Giammario Calia¹  PierAndrea Serra¹  Paola Arrigo¹  Gianfranco Bazzu¹ 

[1] Medical, Surgical and Experimental Sciences, University of Sassari, Viale San Pietro 43/b, 07100 Sassari, Italy;

关键词: glucose;    lactate;    biosensors;    low-temperature storage;    over-time stability;   

DOI  :  10.3390/s19020422

来源: DOAJ

【 摘 要 】

Molecular biomarkers are very important in biology, biotechnology and even in medicine, but it is quite hard to convert biology-related signals into measurable data. For this purpose, amperometric biosensors have proven to be particularly suitable because of their specificity and sensitivity. The operation and shelf stability of the biosensor are quite important features, and storage procedures therefore play an important role in preserving the performance of the biosensors. In the present study two different designs for both glucose and lactate biosensor, differing only in regards to the containment net, represented by polyurethane or glutharaldehyde, were studied under different storage conditions (+4, −20 and −80 °C) and monitored over a period of 120 days, in order to evaluate the variations of kinetic parameters, as V_MAX and K_M, and LRS as the analytical parameter. Surprisingly, the storage at −80 °C yielded the best results because of an unexpected and, most of all, long-lasting increase of V_MAX and LRS, denoting an interesting improvement in enzyme performances and stability over time. The present study aimed to also evaluate the impact of a short-period storage in dry ice on biosensor performances, in order to simulate a hypothetical preparation-conservation-shipment condition.

【 授权许可】

Unknown