Serum-Based Proteomics Reveals Lipid Metabolic and Immunoregulatory Dysregulation in Cervical Artery Dissection With Stroke

Frontiers in Neurology

Jing Peng¹  Yongtao Yang²  Kangning Chen³  Zhenhua Zhou³  Hong Ran³  Suxia Wang³  Jialu Huang³ 

[1] Department of Geriatrics, Sichuan Second Hospital of Traditional Chinese Medicine, Chengdu, China;Department of Neurology, Chongqing Renji Hospital, University of Chinese Academy of Sciences, Chongqing, China;Department of Neurology, Southwest Hospital, Third Military Medical University, Chongqing, China;

关键词: cervical artery dissection;    stroke;    serum;    immunoregulation;    lipid metabolism;   

DOI  :  10.3389/fneur.2020.00352

来源: DOAJ

【 摘 要 】

Cervical artery dissection (CAD) is an important causal factor for stroke in young and middle-aged individuals and presents a great burden to the individual stroke victim. However, the pathophysiological mechanisms underlying CAD remain unknown. Here, an iTRAQ (isobaric tagging for relative and absolute quantitation)–based quantitative proteomic approach was performed, to identify differentially expressed proteins in serum samples obtained from spontaneous CAD and non-CAD ischemic stroke subjects. Differential protein expression was analyzed for Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway overrepresentation, and six differential proteins were selected for enzyme-linked immunosorbent assay validation. Through KEGG analysis, the significantly differentiated proteins were primarily involved in immunoregulation, blood coagulation, and lipid metabolism. For the first time, differential expressions of apolipoprotein B, apolipoprotein C-I, lipopolysaccharide-binding protein, vascular cell adhesion molecule 1, fibulin-1, and ficolin-2 were confirmed as being significantly upregulated in CAD as compared to non-CAD ischemic stroke subjects. In conclusion, proteomic analysis reveals that early perturbation of immunoregulation and lipid metabolism may be involved in the pathophysiology of CAD. Specifically, the panel of six proteins identified is promising as serum-based biomarkers for the detection of increased CAD risk in stroke subjects.

【 授权许可】

Unknown