Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules

Micromachines

Yi Xing¹  Yi Qiao²  Naiyun Long²  Yuhan Luo²  Jing Tu² 

[1] Institute of Child and Adolescent Health, School of Public Health, Peking University, Beijing 100191, China;State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;

关键词: FRET;    smFRET;    biomolecule;    nucleic acid structure;    protein;   

DOI  :  10.3390/mi12050492

来源: DOAJ

【 摘 要 】

Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules.

【 授权许可】

Unknown