Extended spectrum β lactamase-producing Enterobacteriaceae shedding by race horses in Ontario, Canada

BMC Veterinary Research

Shiri Navon-Venezia¹  Holly Archer²  Scott J. Weese²  Wiessam Abu Ahmad³  Israel Nissan⁴  Gal Zizelski Valenci⁴  Darryl Bonder⁵  Yossi Paitan⁶  Erez Hanael⁷  Anat Shnaiderman-Torban⁷  Amir Steinman⁷ 

[1] Department of Molecular Biology, Faculty of Natural Science, Ariel University;Department of Pathobiology, Ontario Veterinary College, University of Guelph;Hadassah Braun School of Public Health and Community Medicine, The Hebrew University of Jerusalem;Ministry of Health, National Public Health Laboratory;Ontario Equine Hospital;Sackler Faculty of Medicine, Department of Clinical Microbiology and Immunology, Tel Aviv University;The Robert H. Smith Faculty of Agriculture, Food and Environment, Koret School of Veterinary Medicine, The Hebrew University of Jerusalem;

关键词: ESBL;    Thoroughbred race horse;    CTX-M;   

DOI  :  10.1186/s12917-020-02701-z

来源: DOAJ

【 摘 要 】

Abstract Background We aimed to investigate the prevalence, molecular epidemiology and prevalence factors for Extended Spectrum β-Lactamase-producing Enterobacteriaceae (ESBL-E) shedding by race horses. A cross-sectional study was performed involving fecal samples collected from 169 Thoroughbred horses that were housed at a large racing facility in Ontario, Canada. Samples were enriched, plated on selective plates, sub-cultured to obtain pure cultures and ESBL production was confirmed. Bacterial species were identified and antibiotic susceptibility profiles were assessed. E. coli sequence types (ST) and ESBL genes were determined using multilocus sequence type (MLST) and sequencing. Whole genome sequencing was performed to isolates harboring CTX-M-1 gene. Medical records were reviewed and associations were investigated. Results Adult horses (n = 169), originating from 16 different barns, were sampled. ESBL-E shedding rate was 12% (n = 21/169, 95% CI 8–18%); 22 ESBL-E isolates were molecularly studied (one horse had two isolates). The main species was E. coli (91%) and the major ESBL gene was CTX-M-1 (54.5%). Ten different E. coli STs were identified. Sixty-four percent of total isolates were defined as multi-drug resistant. ESBL-E shedding horses originated from 8/16 different barns; whereas 48% (10/21) of them originated from one specific barn. Overall, antibiotic treatment in the previous month was found as a prevalence factor for ESBL-E shedding (p = 0.016, prevalence OR = 27.72, 95% CI 1.845–416.555). Conclusions Our findings demonstrate the potential diverse reservoir of ESBL-E in Thoroughbred race horses. Multi-drug resistant bacteria should be further investigated to improve antibiotic treatment regimens and equine welfare.

【 授权许可】

Unknown