Epidemiological distribution of genotypes of Giardia duodenalis in humans in Spain

Parasites & Vectors

Dawn M. Roellig¹  Yaqiong Guo²  Lihua Xiao²  Jordi Huguet³  Olga Gonzalez-Moreno³  Laura Oliver³  Yaoyu Feng⁴  Yuanfei Wang⁴ 

[1] Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention;Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural University;Laboratory of Microbiology and Parasitology, SYNLAB;State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology;

关键词: Giardia duodenalis;    Multilocus genotyping;    Assemblage;    Spain;   

DOI  :  10.1186/s13071-019-3692-4

来源: DOAJ

【 摘 要 】

Abstract Background Although the distribution of Giardia duodenalis genotypes in humans has been increasingly reported in recent years, data on possible differences in pathogen transmission between age groups and virulence between genotypes are scarce. The purpose of this study is to investigate the genetic diversity of G. duodenalis in humans in Spain and compare the distribution of G. duodenalis assemblages A and B between children and adults and clinical presentations between the two genotypes. Methods In the present study, 125 microscopy-positive fecal samples were collected from humans in Spain over a 7-year period. PCR and sequence analyses of the triosephosphate isomerase, β-giardin and glutamate dehydrogenase genes were used to identify the multilocus genotypes of G. duodenalis. Results Sequence analysis of three genetic loci identified both G. duodenalis assemblages A (29) and B (66), with co-infections of the two in two patients. Among the sequences obtained in this study, four multilocus genotypes (MLGs) of the sub-assemblage AII were observed within assemblage A. In contrast, 19 MLGs were detected within assemblage B due to the high sequence diversity at each locus. One MLG, however, was found in 51.9% (27/52) of assemblage B samples. Children were more commonly infected by assemblage B (44/53 or 83%) than adults (22/42 or 52.4%; χ 2 = 10.371, df = 1, P = 0.001). Asymptomatic infection was more common in patients with assemblage A (4/29 or 13.8%) than in those with assemblage B (1/66 or 1.5%; χ 2 = 6.091, df = 1, P = 0.029), and the frequency of abdominal pain occurrence was higher in assemblage B patients (65/66 or 98.5%) than assemblage A patients (25/29 or 86.2%; χ 2 = 6.091, df = 1, P = 0.029). Conclusions These results illustrate the existence of differences in genotype distribution between children and adults and clinical presentations between G. duodenalis genotypes. They are useful in understanding the transmission of G. duodenalis in humans in Spain.

【 授权许可】

Unknown