Purification of polyclonal IgG specific for Camelid’s antibodies and their recombinant nanobodies

Open Life Sciences

Soukkarieh Chadi¹  Haddad Muhammad¹  Abbady Abdul Qader²  Khalaf Houssam Eddin² 

[1] Department of Animal Biology, Faculty of Sciences, Damascus University, Syrian Republic;Department of Molecular Biology and Biotechnology, Atomic Energy Commission of Syria (AECS), Damascus, Syrian Republic;

关键词: camel;    nanobody;    heavy chain antibody;    polyclonal antibody;    affinity chromatography;   

DOI  :  10.1515/biol-2016-0001

来源: DOAJ

【 摘 要 】

Camelid’ s heavy-chain antibody (HCAb) consists of only two heavy chains and lacks the two light chains together with the CH1 domain usually found in conventional immunoglobulins. A recombinant single antigen-binding entity, named VHH (or Nanobody®) was generated by reengineering the variable domains from HCAb. This study focuses on the detection of camelid´s immunoglobulins as well as their derivative nanobodies using a universal anti-camel antibody produced in rabbit (rIgG). Starting from a crude rabbit serum, a standard stock of rIgG (1 mg/ml) was prepared after purification by affinity chromatography using protein-A column. As expected, rIgG was able to detect camel antibodies in ELISA and immunoblotting, and its reactivity was equal against all different camel IgG subclasses, which were purified from serum by differential affinity chromatography on protein-G and -A. Interestingly, rIgG also recognized nanobodies since they were originally part of camel HCAbs, providing an alternative method to detect the corpus of these recombinant proteins rather than targeting their artificial tags. These data suggest that the anti-camel rIgG described here could be efficiently applied at different stages of nanobody technology, including the quantitation of the issued nanobodies and their detection when bound to target antigens.

【 授权许可】

Unknown