The Effects of Exercise and Restriction of Sugar-Sweetened Beverages on Muscle Function and Autophagy Regulation in High-Fat High-Sucrose-Fed Obesity Mice

Diabetes & Metabolism Journal

Liang Tang¹  Didi Zhang²  Ji Hyun Lee²  Seong Eun Kwak²  Hyung Eun Shin²  Wook Song²  Jun Hyun Bae² 

[1] Institute of Sports Biology, Shaanxi Normal University, Xi’an, China;Institute of Sports Science, Seoul National University, Seoul, Korea;

关键词: autophagy;    diet, high-fat;    muscle, skeletal;    obesity;    sucrose;    sugar-sweetened beverages;   

DOI  :  10.4093/dmj.2020.0157

来源: DOAJ

【 摘 要 】

Background Autophagy maintains muscle mass and healthy skeletal muscles. Several recent studies have associated sugar-sweetened beverage (SSB) consumption with diseases. We investigated whether muscle dysfunction due to obesity could be restored by SSB restriction (SR) alone or in combination with exercise (EX) training. Methods Obese mice were subjected to SR combined with treadmill EX. Intraperitoneal glucose tolerance test, grip strength test, hanging time test, and body composition analysis were performed. Triglyceride (TG) and total cholesterol (TC) serum concentrations and TG concentrations in quadriceps muscles were analyzed. Western blot and reverse transcription-quantitative polymerase chain reaction helped analyze autophagy-related protein and mRNA expression, respectively. Results SR alone had no significant effect on fasting blood glucose levels, glucose tolerance, and muscle function. However, it had effect on serum TC, serum TG, and BCL2 interacting protein 3 expression. SR+EX improved glucose tolerance and muscle function and increased serum TC utilization than SR alone. SR+EX reduced P62 levels, increased glucose transporter type 4 and peroxisome proliferator-activated receptor γ coactivator-1α protein expression, and improved grip strength relative to the high-fat and high-sucrose liquid (HFHS) group, and this was not observed in the HFHS+EX group. Conclusion SR induced mitophagy-related protein expression in quadriceps, without affecting muscle function. And, the combination of SR and EX activated mitophagy-related proteins and improved muscle function.

【 授权许可】

Unknown