Promising Antibiofilm Agents: Recent Breakthrough against Biofilm Producing Methicillin-Resistant Staphylococcus aureus

Antibiotics

WesamH. Abdulaal¹  MahmoudM. Bendary²  WaelA. H. Hegazy³  El-sayed Y. El-Naenaeey⁴  MarwaI. Abd El-Hamid⁴  NabilA. Alhakamy⁵  RashaA. Mosbah⁶  MajedS. Nassar⁷  MuhammedA. Bakhrebah⁷  Toka M kandeel⁸ 

[1] Department of Biochemistry, Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Department of Microbiology and Immunology, Faculty of Pharmacy, Port Said University, Port Said 42511, Egypt;Department of Microbiology and Immunology, Faculty of Pharmacy, Zagazig University, Zagazig 44511, Egypt;Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt;Department of Pharmaceutics, Faculty of Pharmacy, King Abdulaziz University, Jeddah 21589, Saudi Arabia;Fellow Pharmacist, Infection Control Unit, Zagazig University Hospital, Zagazig 44511, Egypt;Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086 Riyadh 11442, Saudi Arabia;Specialist of Laboratory Medical Analysis, Almokhtabar Private Laboratories, Zagazig 44511, Egypt;

关键词: MRSA;    antibiofilm;    antimicrobial;    ZnO-NPs;    proteinase-K;    HAM;   

DOI  :  10.3390/antibiotics9100667

来源: DOAJ

【 摘 要 】

Multidrug resistant (MDR) methicillin-resistant Staphylococcus aureus (MRSA) is a superbug pathogen that causes serious diseases. One of the main reasons for the lack of the effectiveness of antibiotic therapy against infections caused by this resistant pathogen is the recalcitrant nature of MRSA biofilms, which results in an increasingly serious situation worldwide. Consequently, the development of innovative biofilm inhibitors is urgently needed to control the biofilm formation by this pathogen. In this work, we thus sought to evaluate the biofilm inhibiting ability of some promising antibiofilm agents such as zinc oxide nanoparticles (Zno NPs), proteinase K, and hamamelitannin (HAM) in managing the MRSA biofilms. Different phenotypic and genotypic methods were used to identify the biofilm producing MDR MRSA isolates and the antibiofilm/antimicrobial activities of the used promising agents. Our study demonstrated strong antibiofilm activities of ZnO NPs, proteinase K, and HAM against MRSA biofilms along with their transcriptional modulation of biofilm (intercellular adhesion A, icaA) and quorum sensing (QS) (agr) genes. Interestingly, only ZnO NPs showed a powerful antimicrobial activity against this pathogen. Collectively, we observed overall positive correlations between the biofilm production and the antimicrobial resistance/agr genotypes II and IV. Meanwhile, there was no significant correlation between the toxin genes and the biofilm production. The ZnO NPs were recommended to be used alone as potent antimicrobial and antibiofilm agents against MDR MRSA and their biofilm-associated diseases. On the other hand, proteinase-K and HAM can be co-administrated with other antimicrobial agents to manage such types of infections.

【 授权许可】

Unknown