Lead acetate toxicity on human lymphocytes at non-cytotoxic concentrations detected in human blood

Main Group Metal Chemistry

Aghvami Marjan¹  Pourahmad Jalal¹  Soodi Maliheh²  Zarei Mohammad Hadi³  Nassireslami Ehsan³ 

[1] Department of Pharmacology and Toxicology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran 19919-53381, Iran;Faculty of Medical Sciences, Department of Toxicology, Tarbiat Modares University, Tehran 00982182884555, Iran;School of Medicine, AJA University of Medical Sciences, Tehran 00982144266273, Iran;

关键词: cytotoxicity;    human lymphocyte;    immune system;    lead acetate;    lysosomal membrane destabilization;    mitochondrial membrane potential;   

DOI  :  10.1515/mgmc-2017-0023

来源: DOAJ

【 摘 要 】

Lead (Pb) is one of the most important heavy metals that possess many applications in different kinds of industrial procedures and consumer products. The adverse effects of Pb on different parts of the immune system have been reported in various studies. Although it has been shown that high concentrations of Pb have low cytotoxicity on human lymphocytes, the effects of non-cytotoxic concentrations of Pb (detected in human blood) on cellular organelles and oxidative stress factors of human lymphocytes have yet to be determined. In this study, human lymphocytes were obtained from the blood of healthy male volunteers through the use of the Ficoll standard method. The intention of this paper was to determine the effects of non-cytotoxic concentrations of Pb on human lymphocytes using the accelerated cytotoxicity mechanism screening technique. For determination of cell viability, lymphocytes were treated with 0–1 mm Pb for 12 h. Subsequently, we assayed the effects of non-cytotoxic concentrations of Pb which are detected in human blood on human lymphocytes and investigated if Pb can affect oxidative stress and cellular organelles at these concentrations. So, in concentrations which have no cytotoxic effects, Pb is shown to induce oxidative stress in addition to inflicting damage on mitochondria and lysosomes in human blood lymphocytes.

【 授权许可】

Unknown