Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity

eLife

Jason Xin¹  Madelen M Díaz¹  Matthias Schlichting¹  Michael Rosbash¹ 

[1] Department of Biology, Howard Hughes Medical Institute, Brandeis University, Waltham, United States;

关键词: circadian rhythm;    network communication;    activity;   

DOI  :  10.7554/eLife.48301

来源: DOAJ

【 摘 要 】

Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light.

【 授权许可】

Unknown