Immunoregulatory activity of a low-molecular-weight heteropolysaccharide from Ganoderma leucocontextum fruiting bodies in vitro and in vivo

Food Chemistry: X

Zhongzheng Chen¹  Chun Xiao¹  Jiayi Qi²  Shaodan Chen²  Bin Li²  Ranhua Zeng²  Chi-Tang Ho³  Huiping Hu⁴  Yizhen Xie⁴  Qingping Wu⁴  Manjun Cai⁴  Xiong Gao⁴ 

[1] Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods, South China Agricultural University, Guangzhou 510642, China;College of Food Science, South China Agricultural University, 483 Wushan Street, Tianhe District, Guangzhou 510642, China;Department of Food Science, Rutgers University, 65 Dudley Road, New Brunswick, NJ 08901, USA;State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Safety and Health, Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, China;

关键词: Ganoderma leucocontextum;    Heteropolysaccharide;    Immunostimulatory activity;    Molecular mechanism;    Immunosuppressed mice;   

DOI  :  

来源: DOAJ

【 摘 要 】

The chemical structure of GLP-1, a novel water-soluble heteropolysaccharide purified Ganoderma leucocontextum fruiting bodies, has been characterized in our previous study. This study aimed to investigate the immunostimulatory activity of GLP-1 in vitro and in vivo by using RAW264.7 macrophages and cyclophosphamide-induced immunosuppressed mice model. Results showed that GLP-1 was able to enhance phagocytic activity and promote the production of reactive oxygen species, nitric oxide, tumor necrosis factor-α, interleukin-6, and monocyte chemoattractant protein-1 in RAW264.7 macrophages. Moreover, GLP-1 could activate mitogen-activated protein kinase, phosphatidylinositol-3-kinase/protein kinase B, and nuclear factor-kappa B signaling pathways through toll-like receptor 2 and dectin-1 receptors. Furthermore, GLP-1 increased the thymus index, serum immunoglobulin levels, and percentage of CD3+ T lymphocytes in cyclophosphamide-induced immunosuppressed mice. These results demonstrated that GLP-1 possessed significant immunostimulatory effects in vivo and in vitro and could be developed as an effective immunomodulator for application in functional foods.

【 授权许可】

Unknown