RT-qPCR Testing of SARS-CoV-2: A Primer

International Journal of Molecular Sciences

Tania Nolan¹  StephenA. Bustin² 

[1] Institute of Population Health, Faculty of Medical and Human Sciences, University of Manchester;Medical Technology Research Centre, Faculty of Health, Education, Medicine and Social Care, Anglia Ruskin University, Chelmsford, Essex CM1 1SQ, UK;

关键词: COVID-19;    SARS;    pandemic;    reverse transcription;    real-time fluorescence PCR;   

DOI  :  10.3390/ijms21083004

来源: DOAJ

【 摘 要 】

Testing for the presence of coronavirus is an essential diagnostic tool for monitoring and managing the current COVID-19 pandemic. The only reliable test in current use for testing acute infection targets the genome of SARS-CoV-2, and the most widely used method is quantitative fluorescence-based reverse transcription polymerase chain reaction (RT-qPCR). Despite its ubiquity, there is a significant amount of uncertainty about how this test works, potential throughput and reliability. This has resulted in widespread misrepresentation of the problems faced using this test during the current COVID-19 epidemic. This primer provides simple, straightforward and impartial information about RT-qPCR.

【 授权许可】

Unknown