The Instrumentation of a Microfluidic Analyzer Enabling the Characterization of the Specific Membrane Capacitance, Cytoplasm Conductivity, and Instantaneous Young’s Modulus of Single Cells

International Journal of Molecular Sciences

Rong Long¹  Min-Hsien Wu²  Chia-Hsun Hsieh²  Yang Zhao³  Chengjun Huang³  Ke Wang⁴  Jian Chen⁴  Junbo Wang⁴  Deyong Chen⁴  Beiyuan Fan⁴ 

[1] Department of Mechanical Engineering, University of Colorado, Boulder, CO 80309, USA;Division of Haematology/Oncology, Department of Internal Medicine, Chang Gung Memorial Hospital at Linkou, Taoyuan City 33302, Taiwan;Institute of Microelectronics of Chinese Academy of Sciences, Beijing 100029, China;State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China;

关键词: instrumentation;    microfluidics;    single-cell analysis;    specific membrane capacitance;    cytoplasm conductivity;    instantaneous Young’s modulus;   

DOI  :  10.3390/ijms18061158

来源: DOAJ

【 摘 要 】

This paper presents the instrumentation of a microfluidic analyzer enabling the characterization of single-cell biophysical properties, which includes seven key components: a microfluidic module, a pressure module, an imaging module, an impedance module, two LabVIEW platforms for instrument operation and raw data processing, respectively, and a Python code for data translation. Under the control of the LabVIEW platform for instrument operation, the pressure module flushes single cells into the microfluidic module with raw biophysical parameters sampled by the imaging and impedance modules and processed by the LabVIEW platform for raw data processing, which were further translated into intrinsic cellular biophysical parameters using the code developed in Python. Based on this system, specific membrane capacitance, cytoplasm conductivity, and instantaneous Young’s modulus of three cell types were quantified as 2.76 ± 0.57 μF/cm2, 1.00 ± 0.14 S/m, and 3.79 ± 1.11 kPa for A549 cells (ncell = 202); 1.88 ± 0.31 μF/cm2, 1.05 ± 0.16 S/m, and 3.74 ± 0.75 kPa for 95D cells (ncell = 257); 2.11 ± 0.38 μF/cm2, 0.87 ± 0.11 S/m, and 5.39 ± 0.89 kPa for H460 cells (ncell = 246). As a semi-automatic instrument with a throughput of roughly 1 cell per second, this prototype instrument can be potentially used for the characterization of cellular biophysical properties.

【 授权许可】

Unknown