Serum Creatine Kinase as a Biomarker to Predict Wooden Breast in vivo for Chicken Breeding

Frontiers in Physiology

Dawei Liu¹  Dan Zhu¹  Xicai Wang³  Fuli Kong³  Zhengxiao He³  Ranran Liu³  Jiahong Sun³  Jie Wen³  Guiping Zhao³ 

[1] Foshan Gaoming Xinguang Agricultural and Animal Industrials Corporation, Foshan, China;Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China;State Key Laboratory of Animal Nutrition, Key Laboratory of Animal (Poultry), Genetics Breeding and Reproduction, Ministry of Agriculture, Beijing, China;

关键词: creatine kinase;    biomarker;    predict;    wooden breast;    in vivo;    chicken breeding;   

DOI  :  10.3389/fphys.2021.711711

来源: DOAJ

【 摘 要 】

The present study aimed to find a blood marker for the prediction of wooden breast (WB) in live broiler to assist the genetic selection of fast-growing chickens. The experiments were carried out with two chicken flocks: 250 male broilers in flock 1 and 100 male and female broilers in flock 2. Both flocks were slaughtered and measured. The breast filets were assessed by combining subjective scoring and compression force at 28 (flock 1 only) and 42 days of age. The enzyme activity in serum and breast tissue (flock 1 only) of normal and affected groups was tested. The results showed that the compression force was significantly different between the normal and affected groups at 28 and 42 days of age (P < 0.001), and it increased significantly with rising WB and WS scores. The serum creatine kinase (CK) value increased significantly with rising compression force at 42 days of age (P < 0.001). The serum CK positively correlated with compression force (r = 0.608; P < 0.001) and the linear regression equation (serum CK = 0.9960∗compression force + 1.884) was established for the line studied. The association between serum CK and compression force is consistent between flocks 1 and 2. In conclusion, our study confirmed that compression force could be the quantitative indicator to differentiate breast filets and found that serum CK could be a candidate biomarker to predict WB in live broilers and assist genetic selection in broiler breeding.

【 授权许可】

Unknown