The fecal microbiota composition of boar Duroc, Yorkshire, Landrace and Hampshire pigs

Asian-Australasian Journal of Animal Sciences

Yun Xiang¹  Weidong Zhou²  Kaifeng Li³  Guohong Gui³  Yingping Xiao³  Hua Yang³ 

[1] Animal Husbandry and Veterinary Institute, Jinhua Academy of Agricultural Sciences, Jinhua 321017, China;Animal Husbandry and Veterinary Institute, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;

关键词: Boar;    Breed;    Fecal Microbiota;    High-throughput Sequencing;    Short-chain Fatty Acids;   

DOI  :  10.5713/ajas.16.0746

来源: DOAJ

【 摘 要 】

Objective To investigate the effect of host genetics on gut microbial diversity, we performed a structural survey of the fecal microbiota of four purebred boar pig lines: Duroc, Landrace, Hampshire, and Yorkshire. Methods The V3–V4 regions of the 16S rRNA genes were amplified and sequenced. Results A total of 783 operational taxonomic units were shared by all breeds, whereas others were breed-specific. Firmicutes and Bacteroidetes dominated the majority of the fecal microbiota; Clostridia, Bacilli, and Bacteroidia were the major classes. Nine predominant genera were observed in all breeds and eight of them can produce short-chain fatty acids. Some bacteria can secrete cellulase to aid fiber digestion by the host. Butyric, isobutyric, valeric, and isovaleric acid levels were highest in Landrace pigs, whereas acetic and propionic acid were highest in the Hampshire breed. Heatmap was used to revealed breed-specific bacteria. Principal coordinate analysis of fecal bacteria revealed that the Landrace and Yorkshire breeds had high similarity and were clearly separated from the Duroc and Hampshire breeds. Conclusion Overall, this study is the first time to compare the fecal microbiomes of four breeds of boar pig by high-throughput sequencing and to use Spearman’s rank correlation to analyze competition and cooperation among the core bacteria.

【 授权许可】

Unknown