Protein Core Fucosylation Regulates Planarian Head Regeneration via Neoblast Proliferation

Frontiers in Cell and Developmental Biology

Yang Yang¹  Yuan Yu¹  Fulin Chen¹  Hongbo Liu²  Jing Zhang²  Hanxue Zheng²  Liyuan Jia²  Wenjun Wang²  Xue Wang² 

[1] Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, School of Medicine, Northwest University, Xi’an, China;Lab of Tissue Engineering, College of Life Sciences, Northwest University, Xi’an, China;Provincial Key Laboratory of Biotechnology of Shaanxi, Northwest University, Xi’an, China;

关键词: planarian;    head regeneration;    neoblast proliferation;    FUT8;    core fucose-binding glycoproteins;   

DOI  :  10.3389/fcell.2021.625823

来源: DOAJ

【 摘 要 】

Protein glycosylation is an important posttranslational modification that plays a crucial role in cellular function. However, its biological roles in tissue regeneration remain interesting and primarily ambiguous. In this study, we profiled protein glycosylation during head regeneration in planarian Dugesia japonica using a lectin microarray. We found that 6 kinds of lectins showed increased signals and 16 kinds showed decreased signals. Interestingly, we found that protein core fucosylation, manifested by Lens culinaris agglutinin (LCA) staining, was significantly upregulated during planarian head regeneration. Lectin histochemistry indicated that the LCA signal was intensified within the wound and blastemal areas. Furthermore, we found that treatment with a fucosylation inhibitor, 2F-peracetyl-fucose, significantly retarded planarian head regeneration, while supplement with L-fucose could improve DjFut8 expression and stimulate planarian head regeneration. In addition, 53 glycoproteins that bound to LCA were selectively isolated by LCA-magnetic particle conjugates and identified by LC-MS/MS, including the neoblast markers DjpiwiA, DjpiwiB, DjvlgA, and DjvlgB. Overall, our study provides direct evidence for the involvement of protein core fucosylation in planarian regeneration.

【 授权许可】

Unknown