The Effects of Three Chlorhexidine-Based Mouthwashes on Human Osteoblast-Like SaOS-2 Cells. An In Vitro Study

International Journal of Molecular Sciences

Gordon John¹  Luisa Scotti¹  Giulia Brunello¹  Jürgen Becker¹  Kathrin Becker²  Dieter Drescher² 

[1] Department of Oral Surgery, University Clinic of Düsseldorf, 40225 Düsseldorf, Germany;Department of Orthodontics, University Clinic of Düsseldorf, 40225 Düsseldorf, Germany;

关键词: antiseptic;    bone;    cetylpyridinium chloride;    chlorhexidine;    mouthrinse;    peri-implantitis;   

DOI  :  10.3390/ijms22189986

来源: DOAJ

【 摘 要 】

Several decontamination methods for removing biofilm from implant surfaces during surgical peri-implantitis treatment have been reported, including the intraoperative usage of chlorhexidine (CHX)-based antiseptics. There is a lack of information on possible adverse effects on bone healing. The study aimed to examine the impact of three CHX-based mouthwashes on osteoblast-like cells (SaOS-2) in vitro. Cells were cultured for three days in 96-well binding plates. Each well was randomly treated for either 30, 60 or 120 s with 0.05% CHX combined with 0.05% cetylpyridinium chloride (CPC), 0.1% CHX, 0.2% CHX or sterile saline (NaCl) as control. Cell viability, cytotoxicity and apoptosis were assessed at day 0, 3 and 6. Cell viability resulted in being higher in the control group at all time points. At day 0, the CHX 0.2 group showed significantly higher cytotoxicity values compared to CHX 0.1 (30 s), CHX + CPC (30 s, 60 s and 120 s) and control (60 s and 120 s), while no significant differences were identified between CHX + CPC and both CHX 0.1 and NaCl groups. All test mouthwashes were found to induce apoptosis to a lower extent compared to control. Results indicate that 0.2% CHX presented the highest cytotoxic effect. Therefore, its intraoperative use should be carefully considered.

【 授权许可】

Unknown