Zika Virus Envelope Protein Domain III Produced in K. phaffii Has the Potential for Diagnostic Applications

Diagnostics

Júlia Maria Alves Meira¹  João Pedro Cruz Colombari¹  John Willians Oliveira Prates²  João Vitor da Silva Rodrigues²  Cynthia Canedo da Silva²  Roberto Sousa Dias³  Mariana Fonseca Xisto³  e Sérgio Oliveira de Paula³ 

[1] Department of Medicine and Nursing, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil;Department of Microbiology, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil;Laboratory of Molecular Immunovirology, Department of General Biology, Federal University of Viçosa, Viçosa 36570-900, Minas Gerais, Brazil;

关键词: Zika virus;    protein E;    domain III;    Komagataella phaffii;    diagnostic;   

DOI  :  10.3390/diagnostics12051198

来源: DOAJ

【 摘 要 】

Zika virus (ZIKV) represents a global human health threat and it is related to severe diseases such as congenital Zika syndrome (CZS) and Guillain-Barré syndrome (GBS). There is no vaccine available nor specific antiviral treatment, so developing sensitive, specific, and low-cost diagnostic tests is necessary. Thus, the objective of this work was to produce the Zika virus envelope protein domain III (ZIKV-EDIII) in Komagataella phaffii KM71H and evaluate its potential for diagnostic applications. After the K. phaffii had been transformed with the pPICZαA-ZIKV-EDIII vector, an SDS-PAGE and Western Blot were performed to characterize the recombinant protein and an ELISA to evaluate the antigenic potential. The results show that ZIKV-EDIII was produced in the expected size, with a good purity grade and yield of 2.58 mg/L. The receiver operating characteristic (ROC) curve showed 90% sensitivity and 87.5% specificity for IgM, and 93.33% sensitivity and 82.76% specificity for IgG. The ZIKV-EDIII protein was efficiently produced in K. phaffi, and it has the potential for diagnostic applications.

【 授权许可】

Unknown