Comparative analysis of immune related genes between domestic pig and germ-free minipig

Laboratory Animal Research

Jeong Ho Hwang¹  Ju Young Lee¹  Hoon Taek Lee²  Sang Eun Kim² 

[1] Animal Model Research Group, Korea Institute of Toxicology;Department of Stem Cell and Regenerative Biotechnology, Konkuk University;

关键词: Minipig;    Germ-free facility;    Differentially expressed gene;    Systemic immune activation;   

DOI  :  10.1186/s42826-020-00077-7

来源: DOAJ

【 摘 要 】

Abstract Recently, minipig has been considered as an animal model that is appropriate for human disease model to study toxicology, pharmacology, and xenotransplantation. Nevertheless, minipigs are bred in various environment according to their use. Here, we suggest that minipigs used for research should be bred in well-controlled facility, comparing immune status of pigs raised in different breeding environment. DNA microarray was performed with ear skin and placenta of Landrace domestic pigs (DPs) and Minnesota germ-free minipigs (GPs). Their immune transcriptome was analyzed by gene ontology (GO) annotation database, based on criteria of |log2 fold change| ≥1 with P ≤ 0.05. As a result, we found that immune related genes in the ear skin of DPs were highly activated, compared to GPs. On the other hand, no significant s were found in the placenta. Quantitative real-time PCR (qRT-PCR) was performed in five candidate immune genes. Their fold changes were consistent with the results from DNA microarray (P ≤ 0.05). In conclusion, we experimentally proved that porcine immune system was affected by different breeding environment, suggesting the importance of controlling microbes in animal room for the qualified research.

【 授权许可】

Unknown