期刊论文详细信息
Frontiers in Cell and Developmental Biology
Protein Lysine Acetylation in Ovarian Granulosa Cells Affects Metabolic Homeostasis and Clinical Presentations of Women With Polycystic Ovary Syndrome
Jie Qiao1  Rong Li2  Yang Yu2  Zheying Min2  Hongcui Zhao2  Xiumei Zhen2  Mo Li2  Xiaoyu Long2  Yong Fan4  Yue Zhao8 
[1] Beijing Advanced Innovation Center for Genomics, Beijing, China;Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproductive Technology, Beijing, China;Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China;Key Laboratory for Major Obstetric Diseases of Guangdong Province, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, China;Key Laboratory of Assisted Reproduction, Ministry of Education, Peking University, Beijing, China;National Clinical Research Center for Obstetrics and Gynecology, Peking University Third Hospital, Beijing, China;Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China;Research Units of Comprehensive Diagnosis and Treatment of Oocyte Maturation Arrest, Chinese Academy of Medical Sciences, Beijing, China;
关键词: lysine acetylation;    polycystic ovary syndrome;    granulosa cells;    metabolic pathway;    acetyl-CoA acetyltransferase 1;   
DOI  :  10.3389/fcell.2020.567028
来源: DOAJ
【 摘 要 】

Polycystic ovary syndrome (PCOS) is one of the most common reproductive endocrine disorders accompanied by obvious metabolic abnormalities. Lower-quality oocytes and embryos are often found in PCOS women during assisted reproductive technology treatment. However, there is still no clarity about the mechanism of ovarian metabolic disorders and the impact on oocyte maturation in PCOS. The aim of this study was to understand the potential effect of the posttranslational modification on ovarian metabolic homeostasis and oocyte development potential in women with PCOS. A quantitative analysis of acetylated proteomics in ovarian granulosa cells of PCOS and control groups was carried out by mass spectrometry. There was widespread lysine acetylation of proteins, of which 265 proteins had increased levels of acetylation and 68 proteins had decreased levels of acetylation in the PCOS group. Most notably, differentially acetylated proteins were significantly enriched in the metabolic pathways of glycolysis, fatty acid degradation, TCA cycle, tryptophan metabolism, and branched-chain amino acid degradation. Acetyl-CoA acetyltransferase 1 (ACAT1) was an enzyme central to these metabolic pathways with increased acetylation level in the PCOS group, and there was a negative correlation of ACAT1 acetylation levels in PCOS granulosa cells with oocyte quality and embryo development efficiency in the clinic. Lysine acetylation changes of key enzymes in PCOS granulosa cells might attenuate their activities and alter metabolic homeostasis of follicular microenvironment for oocyte maturation and embryo development.

【 授权许可】

Unknown   

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