Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology

iScience

Marina Walther-Antonio¹  William Herbert²  Jin Jen³  Jean-Pierre de Vera⁴  Patricio Jeraldo⁵  Bruce Eckloff⁵  Mickael Baqué⁶  Yuguang Liu⁷  Samantha McDonough⁷  Thomas Leya⁸  Charles Cockell⁸  Dirk Schulze-Makuch⁹ 

[1] Department of Experimental Limnology, Leibniz Institute of Groundwater Ecology and Inland Fisheries (IGB), 16775 Stechlin, Germany;Department of Immunology, Mayo Clinic, Rochester, MN 55905, USA;GFZ German Research Centre for Geosciences, 14473 Potsdam, Germany;Mayo Clinic Graduate School of Biomedical Sciences, Mayo Clinic, Rochester, MN 55905, USA;Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA;Astrobiology Group, Center for Astronomy and Astrophysics, Technische Universität Berlin, Hardenbergstr. 36, 10623 Berlin, Germany;Department of Surgery, Division of Surgical Research, Mayo Clinic, Rochester, MN 55905, USA;Medical Genome Facility, Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA;Microbiome Program, Center for Individualized Medicine, Mayo Clinic, Rochester, MN 55905, USA;

关键词: Microbiology;    Microbial genomics;    Space sciences;    Astrobiology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: The Nostoc sp. strain CCCryo 231-06 is a cyanobacterial strain capable of surviving under extreme conditions and thus is of great interest for the astrobiology community. The knowledge of its complete genome sequence would serve as a guide for further studies. However, a major concern has been placed on the effects of contamination on the quality of sequencing data without a reference genome. Here, we report the use of microfluidic technology combined with single cell sequencing and de novo assembly to minimize the contamination and recover the complete genome of the Nostoc strain CCCryo 231-06 with high quality. 100% of the whole genome was recovered with all contaminants removed and a strongly supported phylogenetic tree. The data reported can be useful for comparative genomics for phylogenetic and taxonomic studies. The method used in this work can be applied to studies that require high-quality assemblies of genomes of unknown microorganisms.

【 授权许可】

Unknown