Characterization of polysaccharides from different species of brown seaweed using saccharide mapping and chromatographic analysis

BMC Chemistry

Wancong Zhang¹  Shijie Tang¹  Shengqin Chen²  Kit-Leong Cheong²  Malairaj Sathuvan²  Xiao Zhang²  Yang Liu² 

[1] Department of Plastic Surgery and Burn Center, Second Affiliated Hospital, Shantou University Medical College;Guangdong Provincial Key Laboratory of Marine Biotechnology, STU-UNIVPM Joint Algal Research Center, Institute of Marine Sciences, Shantou University;

关键词: Brown seaweed;    Polysaccharide;    Saccharide mapping;    Chromatographic analysis;    Antioxidant activity;   

DOI  :  10.1186/s13065-020-00727-w

来源: DOAJ

【 摘 要 】

Abstract Brown seaweed polysaccharides (BSPs) are one of the primary active components from brown seaweed that has a range of pharmaceutical and biomedical applications. However, the quality control of BSPs is a challenge due to their complicated structure and macromolecule. In this study, saccharide mapping based on high-performance liquid chromatography (HPLC), multi-angle laser light scattering, viscometer, and refractive index detector (HPSEC-MALLS-Vis-RID), and Fourier transform infrared (FT-IR) were used to discriminate the polysaccharides from nine different species of brown algae (BA1-9). The results showed that BSPs were composed of β-D-glucans and β-1,3−1,4-glucan linkages. The molecular weight, radius of gyration, and intrinsic viscosity of BSPs were ranging from 1.718 × 105 Da to 6.630 × 105 Da, 30.2 nm to 51.5 nm, and 360.99 mL/g to 865.52 mL/g, respectively. Moreover, α values of BSPs were in the range of 0.635 to 0.971, which indicated a rigid rod chain conformation. The antioxidant activities of BSPs exhibited substantial radical scavenging activities against DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2, 2’-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid) radicals, which indicated that the use of BSPs might be a potential approach for antioxidant supplements. Thus, this study gives insights about the structure-function relationship of BSPs, which will be beneficial to improve the quality of polysaccharides derived from marine algae.

【 授权许可】

Unknown