Microbial Communities and Sulfate-Reducing Microorganisms Abundance and Diversity in Municipal Anaerobic Sewage Sludge Digesters from a Wastewater Treatment Plant (Marrakech, Morocco)

Processes

Rémy Guyoneaud¹  Magali Ranchou-Peyruse¹  Anthony Ranchou-Peyruse¹  Abdelaziz El Houari¹  MariaSoledad Goni Urriza¹  Radia Bouterfas²  Abdel-Ilah Qatibi²  Rhizlane Bennisse² 

[1]Environmental Microbiology, Université de Pau et des Pays de l’Adour, E2S UPPA, CNRS, IPREM UMR 5254, 64000 Pau, France

[2]Water Biodiversity and Climate Change Laboratory, Semlalia-Sciences Faculty, Cadi Ayyad University, 40 000 Marrakech, Morocco

关键词: anaerobic digestion;    microbial diversity;    sulfate-reducing bacteria;    Archaea;    sulfides;    biogas;   

DOI  :  10.3390/pr8101284

来源: DOAJ

【 摘 要 】

Both molecular analyses and culture-dependent isolation were combined to investigate the diversity of sulfate-reducing prokaryotes and explore their role in sulfides production in full-scale anaerobic digesters (Marrakech, Morocco). At global scale, using 16S rRNA gene sequencing, Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Synergistetes, and Euryarchaeota were the most dominant phyla. The abundance of Archaea (3.1–5.7%) was linked with temperature. The mcrA gene ranged from 2.18 × 10⁵ to 1.47 × 10⁷ gene copies.g⁻¹ of sludge. The sulfate-reducing prokaryotes, representing 5% of total sequences, involved in sulfides production were Peptococcaceae, Syntrophaceae, Desulfobulbaceae, Desulfovibrionaceae, Syntrophobacteraceae, Desulfurellaceae, and Desulfobacteraceae. Furthermore, dsrB gene ranged from 2.18 × 10⁵ to 1.92 × 10⁷ gene copies.g⁻¹ of sludge. The results revealed that exploration of diversity and function of sulfate-reducing bacteria may play a key role in decreasing sulfide production, an undesirable by-product, during anaerobic digestion.

【 授权许可】

Unknown