Ultra-Low Electromagnetic Fields Application on In Vitro Cartilage Regeneration: A Pilot Study to Improve Treatment of Osteoarticular Diseases

Applied Sciences

Giacomo Bagni¹  Annarosa Arcangeli¹  Valentina Devescovi¹  Jessica Iorio¹  Claudia Duranti¹  Roberto Duranti²  Pietro De Biase² 

[1] Department of Experimental and Clinical Medicine, University of Florence, Viale GB Morgagni 50, 50134 Florence, Italy;General Orthopaedics and Traumatology, AOU Careggi, Largo Palagi 1, 50139 Florence, Italy;

关键词: ultra-low electromagnetic fields;    osteoarthritis;    collagen;    adipose-derived stem cells;    cartilage regeneration;    chondrogenesis markers;   

DOI  :  10.3390/app12094116

来源: DOAJ

【 摘 要 】

Extremely low-frequency and low-intensity electromagnetic fields show positive effects on the treatment of several osteoarticular diseases, such as osteoarthritis, and are currently applied in the clinical setting with promising results on tissue regeneration. However, the biological mechanisms underlying the beneficial effects triggered by this type of physical stimulation still need to be deciphered. We tested the hypothesis that ultra-low complex electromagnetic fields stimulation using an innovative medical device could enhance chondrogenesis in human adipose-derived stem cells (ADSCs), and analyzed its biological effects. Chondrogenic lineage markers, like ACAN, SOX9, RUNX2, COL2A1, and COL10A1, were evaluated after 21 days of treatment. Thus far, we have provided preliminary evidence that a dedicated pattern of ultra-weak complex electromagnetic sequences emitted by a cutting-edge technology can promote cartilage regeneration, inducing the chondrogenic differentiation and maturity of ADSCs.

【 授权许可】

Unknown