Antioxidant and anti-diabetic activities of bioactive fractions of Carica papaya seeds extract

Journal of King Saud University: Science

Dluya Thagriki¹  Manal M. Al Shaqha¹  D. Esther Lydia²  Reuben Agada³  Mona S. Alwahibi⁴  Mohamed Soliman Elshikh⁴  Ameer Khusro⁵  Jawaher Alkahtani⁶ 

[1] Corresponding authors.;CSIR-Indian Institute of Chemical Biology, Kolkata, 4, Raja S.C., Mullick Road, Jadavpur, Kolkata 700032, West Bengal, India;Department of Biochemistry, School of Life Sciences, Modibbo Adama University of Technology Yola, Nigeria;Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia;Food Chemistry and Food Processing, Loyola College, Nungambakkam, Chennai 600034, India;Research Department of Plant Biology and Biotechnology, Loyola College, Nungambakkam, Chennai 600034, India;

关键词: Antioxidant;    Carica papaya seeds;    Diabetes mellitus;    Gas chromatography-mass spectrometry;   

DOI  :  

来源: DOAJ

【 摘 要 】

Carica papaya has tremendous medicinal applications against diverse diseases since ancient period. Considering this, the present study was aimed to assess in vitro antioxidant and anti-diabetic activities of bioactive fractions of C. papaya seed extracts. Initially, the shade-dried ripen seeds of C. papaya were powdered and extracted via soxhlet protocol to obtain crude extracts. The crude extracts were then fractionated using different solvents viz. hexane, ethyl acetate, methanol, and water. Fractions obtained were assessed for α-glucosidase and amylase inhibitory activities using standard methodologyies. Results revealed that ethyl acetate fraction C (83.66 ± 0.10%) and D (84.56 ± 0.23%) showed maximum inhibition of α-amylase with an IC50 value of 36.84 and 36.86 mg/ml, respectively. Likewise, ethyl acetate fraction C (48.72 ± 0.10%) and D (51.81 ± 0.45%) exhibited α-glucosidase inhibition with an IC50 value of 83.54 and 82.33 mg/ml, respectively. The ethyl acetate fractions C and D were further purified to obtain sub-fraction K, L, M, and N and evaluated for in vitro antioxidant and anti-diabetic activities. The sub-fraction K demonstrated maximum antioxidant activity against DPPH and TBA radicals (IC50 = 41.37 and 53.98 mg/ml) respectively. However, the sub-fraction K elicited the most conspicuous anti-diabetic activity towards α-amylase (IC50 = 156.15 mg/ml) and intestinal α-glucosidase (IC50 = 70.89 mg/ml). The mode of inhibition was determined using the Lineweaver- Burk plot. Sub-fraction K exhibited a competitive mechanism of inhibition on both α-amylase and α-glucosidase. The GC-MS profile confirmed the presence of diverse metabolites in the sub-fraction and the major bioactive compounds detected were hexadecanoic acid, methyl ester, 11-octadecenoic acid, methyl ester, N, N-dimethyl, n-hexadecanoic acid, and oleic acid. Our findings suggested the folklore medicinal use of C. papaya seeds as an effective antioxidant and anti-diabetic agents.

【 授权许可】

Unknown