Antimicrobial Activity and Antibiofilm Potential of Coenzyme Q0 against Salmonella Typhimurium

Foods

Xinyi Han¹  Zhuokai Yang¹  Ruixia Wang¹  Pengyu Zhao¹  Xiaoyu Ma¹  Chao Shi¹  Ziyi Li²  Huidong Xu²  Yan Li² 

[1] College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China;College of Innovation and Experiment, Northwest A&F University, Yangling 712100, China;

关键词: coenzyme Q₀;    Salmonella Typhimurium;    cell membrane;    biofilm;    raw chicken;   

DOI  :  10.3390/foods10061211

来源: DOAJ

【 摘 要 】

Coenzyme Q₀ (CoQ₀) has anti-inflammatory and anti-tumor effects; however, the antimicrobial and antibiofilm activities of CoQ₀ against Salmonella enterica serovar Typhimurium are unknown. Thus, we investigated the bacteriostatic and antibiofilm activities, along with the underlying mechanism, of CoQ₀ against S. Typhimurium. The minimum inhibitory concentration (MIC) of CoQ₀ against S. enterica serovars Typhimurium was 0.1–0.2 mg/mL (549–1098 µM), and CoQ₀ at MIC and 2MIC decreased viable S. Typhimurium counts below detectable limits within 6 and 4 h, respectively. CoQ₀ at 20MIC (4 mg/mL) reduced S. Typhimurium on raw chicken by 1.5 log CFU/cm³ within 6 h. CoQ₀ effectively disrupted cell membrane integrity and induced morphological changes in the cell, resulting in hyperpolarization, decreased intracellular ATP concentrations, and cellular constituents leakage. Biofilm-associated S. Typhimurium cells were killed by CoQ₀ treatment. These findings suggest that CoQ₀ could be applied as a natural antibacterial substance for use against S. Typhimurium by the food industry.

【 授权许可】

Unknown