Mapping Stem Rust (Puccinia graminis f. sp. secalis) Resistance in Self-Fertile Winter Rye Populations

Frontiers in Plant Science

Anna Tratwal¹  Marek Korbas¹  Viktor Korzun²  Thomas Miedaner³  Hans-Peter Piepho³  Silvia Koch³  Franz-Joachim Fromme⁴  Jakub Danielewicz⁵  Dörthe Siekmann⁵  Kerstin Flath⁶  Anne-Kristin Schmitt⁶  Renata Krysztofik⁷  Karol Marciniak⁷  Małgorzata Niewińska⁷  Andres Gordillo⁸  Malthe Schmidt⁸  Brigitta Schmiedchen⁸  Jakob Eifler⁸  Paul Gruner^1,10 

[1] Co. KG, Schenkenberg, Germany;Co. KGaA, Einbeck, Germany;Danko Hodowla Roślin Sp. z o.o., Kościan, Poland;Federal State Budgetary Institution of Science Federal Research Center “Kazan Scientific Center of Russian Academy of Sciences”, Kazan, Russia;;HYBRO Saatzucht GmbH &Institute for Plant Protection in Field Crops and Grassland, Julius-Kuehn Institute, Kleinmachnow, Germany;Institute of Plant Protection – National Research Institute, Poznań, Poland;KWS LOCHOW GmbH, Bergen, Germany;;KWS SAAT SE &State Plant Breeding Institute, University of Hohenheim, Stuttgart, Germany;

关键词: rust;    all-stage resistance;    adult-plant resistance;    QTL;    mapping;    mixed model;   

DOI  :  10.3389/fpls.2020.00667

来源: DOAJ

【 摘 要 】

Rye stem rust caused by Puccinia graminis f. sp. secalis can be found in all European rye growing regions. When the summers are warm and dry, the disease can cause severe yield losses over large areas. To date only little research was done in Europe to trigger resistance breeding. To our knowledge, all varieties currently registered in Germany are susceptible. In this study, three biparental populations of inbred lines and one testcross population developed for mapping resistance were investigated. Over 2 years, 68–70 genotypes per population were tested, each in three locations. Combining the phenotypic data with genotyping results of a custom 10k Infinium iSelect single nucleotide polymorphism (SNP) array, we identified both quantitatively inherited adult plant resistance and monogenic all-stage resistance. A single resistance gene, tentatively named Pgs1, located at the distal end of chromosome 7R, could be identified in two independently developed populations. With high probability, it is closely linked to a nucleotide-binding leucine-rich repeat (NB-LRR) resistance gene homolog. A marker for a competitive allele-specific polymerase chain reaction (KASP) genotyping assay was designed that could explain 73 and 97% of the genetic variance in each of both populations, respectively. Additional investigation of naturally occurring rye leaf rust (caused by Puccinia recondita ROEBERGE) revealed a gene complex on chromosome 7R. The gene Pgs1 and further identified quantitative trait loci (QTL) have high potential to be used for breeding stem rust resistant rye.

【 授权许可】

Unknown