Loss of Cyclin-Dependent Kinase Inhibitor Alters Oncolytic Adenovirus Replication and Promotes More Efficient Virus Production

Cancers

Naseruddin Höti¹  WasimH. Chowdhury²  Ronald Rodriguez²  TamaraJane Johnson³ 

[1] Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA;Department of Urology, University of Texas Health Science Center San Antonio, San Antonio, TX 78229, USA;James Brady Urological Institute, The Johns Hopkins School of Medicine, Baltimore, MD 21205, USA;

关键词: adenovirus replication;    p21;    oncolytic adenovirus;   

DOI  :  10.3390/cancers10060202

来源: DOAJ

【 摘 要 】

We elucidate the role of p21/Waf-1, a cyclin-dependent kinase inhibitor, on the oncolytic infection and replication cycle of adenovirus by studying both mRNA and adenoviral proteins expression. We found that infection in the absence of p21 causes a significant increase in adenoviral genomes and late gene expression. Similarly, the oncolytic adenoviral infected p21−/− cells have earlier formation of replication foci and robust replication kinetics that were not observed in the wild type p21/Waf-1 intact cells. These findings suggest a culmination that the presence of intact p21 in host cells causes defects in the oncolytic viral life cycle which results in the production of immature and noninfectious particles.

【 授权许可】

Unknown