Lactobacillus reuteri in its biofilm state promotes neurodevelopment after experimental necrotizing enterocolitis in rats

Brain, Behavior, & Immunity - Health

Robert M. Jaggers¹  John R. Buzzo¹  Lauren Mashburn-Warren¹  Steven D. Goodman¹  Michael T. Bailey¹  Adrian Rajab²  Pamela Mar²  Shivani Deshpande²  Yijie Wang²  Jeffrey D. Galley²  Gail E. Besner²  Terri Shaffer³ 

[1] Center for Microbial Pathogenesis, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA;Center for Perinatal Research, The Research Institute at Nationwide Children’s Hospital, Department of Pediatric Surgery, Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA;Preclinical Imaging and Behavior Core/Animal Resources Core, The Research Institute at Nationwide Children’s Hospital, 700 Children’s Drive, Columbus, OH, 43205, USA;

关键词: Necrotizing enterocolitis (NEC);    Cognitive function;    Learning;    Memory;    Probiotics;    Lactobacillus reuteri;   

DOI  :  

来源: DOAJ

【 摘 要 】

Necrotizing enterocolitis (NEC) is a devastating disease affecting premature newborns with no known cure. Up to half of survivors subsequently exhibit cognitive impairment and neurodevelopmental defects. We created a novel probiotics delivery system in which the probiotic Lactobacillus reuteri (Lr) was induced to form a biofilm [Lr (biofilm)] by incubation with dextranomer microspheres loaded with maltose (Lr-DM-maltose). We have previously demonstrated that a single dose of the probiotic Lr administered in its biofilm state significantly reduces the incidence of NEC and decreases inflammatory cytokine production in an animal model of the disease. The aim of our current study was to determine whether a single dose of the probiotic Lr administered in its biofilm state protects the brain after experimental NEC. We found that rat pups exposed to NEC reached developmental milestones significantly slower than breast fed pups, with mild improvement with Lr (biofilm) treatment. Exposure to NEC had a negative effect on cognitive behavior, which was prevented by Lr (biofilm) treatment. Lr administration also reduced anxiety-like behavior in NEC-exposed rats. The behavioral effects of NEC were associated with increased numbers of activated microglia, decreased myelin basic protein (MBP), and decreased neurotrophic gene expression, which were prevented by administration of Lr (biofilm). Our data indicate early enteral treatment with Lr in its biofilm state prevented the deleterious effects of NEC on developmental impairments.

【 授权许可】

Unknown