Organic Microbial Electrochemical Transistor Monitoring Extracellular Electron Transfer

Advanced Science

Eleni Stavrinidou¹  Arghyamalya Roy¹  Xenofon Strakosas¹  Gábor Méhes¹  Daniel T. Simon¹  Magnus Berggren¹ 

[1] Laboratory of Organic Electronics Department of Science and Technology Linköping University Norrköping 60174 Sweden;

关键词: extracellular electron transfer;    microbial electrochemical systems;    organic electrochemical transistors (OECTs);    PEDOT:PSS;    Shewanella oneidensis;   

DOI  :  10.1002/advs.202000641

来源: DOAJ

【 摘 要 】

Abstract Extracellular electron transfer (EET) denotes the process of microbial respiration with electron transfer to extracellular acceptors and has been exploited in a range of microbial electrochemical systems (MESs). To further understand EET and to optimize the performance of MESs, a better understanding of the dynamics at the microscale is needed. However, the real‐time monitoring of EET at high spatiotemporal resolution would require sophisticated signal amplification. To amplify local EET signals, a miniaturized bioelectronic device, the so‐called organic microbial electrochemical transistor (OMECT), is developed, which includes Shewanella oneidensis MR‐1 integrated onto organic electrochemical transistors comprising poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) combined with poly(vinyl alcohol) (PVA). Bacteria are attached to the gate of the transistor by a chronoamperometric method and the successful attachment is confirmed by fluorescence microscopy. Monitoring EET with the OMECT configuration is achieved due to the inherent amplification of the transistor, revealing fast time‐responses to lactate. The limits of detection when using microfabricated gates as charge collectors are also investigated. The work is a first step toward understanding and monitoring EET in highly confined spaces via microfabricated organic electronic devices, and it can be of importance to study exoelectrogens in microenvironments, such as those of the human microbiome.

【 授权许可】

Unknown