NANOS2 suppresses the cell cycle by repressing mTORC1 activators in embryonic male germ cells

iScience

Hiroko Koike¹  Ryuki Shimada²  Takamasa Hirano²  Yumiko Saga²  Yuzuru Kato³ 

[1] Mammalian Development Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan;Department of Genetics, SOKENDAI, Yata 1111, Mishima, Shizuoka 411-8540, Japan;Mammalian Development Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan;

关键词: biological sciences;    molecular biology;    developmental biology;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: During murine germ cell development, male germ cells enter the mitotically arrested G0 stage, which is an initial step of sexually dimorphic differentiation. The male-specific RNA-binding protein NANOS2 has a key role in suppressing the cell cycle in germ cells. However, the detailed mechanism of how NANOS2 regulates the cell cycle remains unclear. Using single-cell RNA sequencing (scRNA-seq), we extracted the cell cycle state of each germ cell in wild-type and Nanos2-KO testes and revealed that Nanos2 expression starts in mitotic cells and induces mitotic arrest. We identified Rheb, a regulator of mTORC1, and Ptma as possible targets of NANOS2. We propose that repression of the cell cycle is a primary function of NANOS2 and that it is mediated via the suppression of mTORC1 activity through the repression of Rheb in a post-transcriptional manner.

【 授权许可】

Unknown