A novel Gynostemma pentaphyllum saponin and its adipogenesis inhibitory effect through modulating Wnt/β-catenin pathway and cell cycle in mitotic clonal expansion

Journal of Functional Foods

Seong-Ho Lee¹  Liangli (Lucy) Yu²  Jie Liu³  Xiangjun Sun³  Puyu Yang³  Haiming Shi³ 

[1] Corresponding author. Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Tel.: [phone];fax: [phone].;Institute of Food and Nutraceutical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China;

关键词: Gynostemma pentaphyllum;    Saponin;    Adipogenesis;    Mitosis clone expansion;    CCAAT/enhancer-binding protein β;    Wnt/β-catenin signaling;   

DOI  :  

来源: DOAJ

【 摘 要 】

A novel saponin (JS) was isolated from the tetraploid jiaogulan, a popular tea also known as Gynostemma pentaphyllum, and characterized for its chemical structure. JS was investigated for its inhibitory effect on adipocyte differentiation and the potential molecular mechanisms using 3T3-L1 murine pre-adipocytes. JS treatment down-regulated the expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα) at both mRNA and protein levels. Further analysis proved that the inhibition was primarily limited to the early stage of adipocyte differentiation, where JS decreased the DNA-binding activity of C/EBPβ by blocking its phosphorylation. In addition, JS increased phosphorylation of glycogen synthase kinase 3β (GSK-3β) and subsequently increased stability and nuclear translocation of β-catenin through modulating expression of Wnt/β-catenin signaling components. Moreover, JS-induced down regulation of adipogenic markers and fat accumulation is β-catenin-dependent. These data suggested that this novel saponin compound might inhibit adipogenesis through activating Wnt/β-catenin signaling during adipocyte differentiation.

【 授权许可】

Unknown