Cytoprotective Activity of p-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells

Molecules

Ekaterina A. Yurchenko¹  Dmitry L. Aminin¹  Ekaterina S. Menchinskaya¹  Evgeny A. Pislyagin¹  Ekaterina A. Chingizova¹  Elena V. Girich²  Anton N. Yurchenko²  Valery V. Mikhailov³ 

[1] Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia;Laboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia;Laboratory of Microbiology, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia;

关键词: marine fungi;    secondary metabolites;    cytoprotection;    reactive oxygen species;    paraquat;    rotenone;   

DOI  :  10.3390/molecules26123618

来源: DOAJ

【 摘 要 】

The influence of p-terphenyl polyketides 1–3 from Aspergillus candidus KMM 4676 and cerebroside flavuside B (4) from Penicillium islandicum (=Talaromyces islandicus) against the effect of neurotoxins, rotenone and paraquat, on Neuro-2a cell viability by MTT and LDH release assays and intracellular ROS level, as well as DPPH radical scavenging activity, was investigated. Pre-incubation with compounds significantly diminished the ROS level in rotenone- and paraquat-treated cells. It was shown that the investigated polyketides 1–3 significantly increased the viability of rotenone- and paraquat-treated cells in two of the used assays but they affected only the viability of paraquat-treated cells in the LDH release assay. Flavuside B statistically increased the viability of paraquat-treated cells in both MTT and LDH release assays, however, it increased the viability of rotenone-treated cells in the LDH release assay. Structure–activity relationships for p-terphenyl derivatives, as well as possible mechanisms of cytoprotective action of all studied compounds, were discussed.

【 授权许可】

Unknown