Self-Interference (SELFI) Microscopy for Live Super-Resolution Imaging and Single Particle Tracking in 3D

Frontiers in Physics

Gregory Giannone¹  Laurent Groc²  Laurent Cognet²  Pierre Bon³  Jeanne Linarès-Loyez³  Olivier Rossier⁴  Joana S. Ferreira⁴  Brahim Lounis⁵ 

[1] CNRS, LP2N UMR 5298, Talence, France;CNRS, IINS UMR 5297, Bordeaux, France;;Institut d'Optique &Interdisciplinary Institute for Neurosciences, UMR 5297, Université de Bordeaux, Bordeaux, France;Laboratoire Photonique Numérique et Nanosciences, UMR 5298, Université de Bordeaux, Talence, France;

关键词: super-resolution (SR) imaging;    single molecule localization microscopy (SMLM);    phase imaging;    interferometry;    fluorescence;    single particle tracking (SPT);   

DOI  :  10.3389/fphy.2019.00068

来源: DOAJ

【 摘 要 】

Through the formation of fluorescent self-interference (SELFI), quantitative intensity and phase imaging enables the 3D localization of single fluorescent molecules inside a fixed tissue with an accuracy well-beyond the diffraction limit. Here we demonstrate that this concept can be extended to 3D super-resolution microscopy and 3D single particle tracking in various living samples ranging from adherent cells to organotypic brain slices, using diverse fluorescent emitters (fluorescent proteins, organic dyes or quantum dots). This basically covers the most popular single molecule imaging techniques used for live sample studies. We also show that SELFI can be used in combination with different illumination schemes including highly inclined illumination and total internal reflection.

【 授权许可】

Unknown