Increased Frequency of Memory CD4+ T-Cell Responses in Individuals With Previously Treated Extrapulmonary Tuberculosis

Frontiers in Immunology

Bruno B. Andrade²  Alexandre Almeida⁵  Beatriz Barreto-Duarte⁵  Jose Roberto Lapa e Silva⁵  Cynthia H. Nochowicz⁹  Christian Warren⁹  Timothy R. Sterling⁹  Louise Barnett⁹  Spyros A. Kalams⁹  Amondrea Blackman⁹  Christina T. Fiske⁹  Rita M. Smith⁹ 

[1] Curso de Medicina, Centro Universitário Faculdades de Tecnologia e Ciências (UniFTC), Salvador, Brazil;Curso de Medicina, Escola Bahiana de Medicina e Saúde Pública, Salvador, Brazil;Division of Infectious Diseases, Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, United States;Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil;Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;Instituto Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Brazil;Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative, Salvador, Brazil;Universidade Salvador (UNIFACS), Laureate Universities, Salvador, Brazil;Vanderbilt Tuberculosis Center, Vanderbilt University Medical Center, Nashville, TN, United States;

关键词: tuberculosis;    extrapulmonary tuberculosis;    memory T-cell responses;    cytokine;    CD4+ T cell;   

DOI  :  10.3389/fimmu.2020.605338

来源: DOAJ

【 摘 要 】

Extrapulmonary TB (EPTB) occurs with increased frequency in persons with underlying immunodeficiency. Even after recovery from acute illness, differences in immune phenotype and activation persist. Studies defining characteristics of immune responses after recovery from extrapulmonary TB may provide insights into factors that increase TB risk. We performed two case-control studies (in the United States and Brazil) among HIV-seronegative adults with previous EPTB (n = 9; 25), previous pulmonary TB (n = 7; 25), latent M. tuberculosis (Mtb) infection (n = 11; 25), and uninfected TB contacts (n = 10; 25). We assessed the frequency of dual CD4+ interferon-γ and tumor necrosis factor-α responses after stimulation with overlapping Mtb peptides from ESAT-6 or CFP-10, or gamma-irradiated Mtb H37Rv, proliferative responses to Mtb antigens, T-regulatory cell (Treg) frequency and phenotype. In both study populations, individuals with prior EPTB had the highest frequency of intracellular cytokine-producing cells in response to Mtb antigens (p < 0.05; p <.0001). Persons with prior EPTB in Brazil had the highest levels of CD4 proliferation to Mtb antigens (p < 0.0001), and the highest expression of CD39 on Tregs (p < 0.0001). Individuals with treated EPTB maintained high frequencies of Mtb-specific memory responses and active Treg cells, suggesting that susceptibility to EPTB occurs despite the ability to develop and maintain enhanced adaptive immune responses.

【 授权许可】

Unknown