Non-Destructive Genotyping of Cultivars and Strains of Sesame through NIR Spectroscopy and Chemometrics

Biosensors

Oscar Smiderle¹  Francisco dos Santos Panero²  João S. Panero³  Pedro dos S. Panero⁴  Anselmo F. R. Rodriguez⁵  Fernando S. D. V. Faria⁵ 

[1] Brazilian Agricultural Research Corporation (Embrapa), Boa Vista 69301-970, RR, Brazil;Chemistry Department, Federal University of Roraima, Boa Vista 69310-000, RR, Brazil;Federal Institute of Science and Technology of Roraima, Campus Boa Vista, Boa Vista 69303-340, RR, Brazil;Federal Institute of Science and Technology of Roraima, Campus Zona Oeste, Boa Vista 69318-000, RR, Brazil;Science and Technology Innovation Program, Federal University of Acre, Rio Branco 69915-900, AC, Brazil;

关键词: Sesamum indicum;    NIR;    PCA;    HCA;    KNN;    SIMCA;   

DOI  :  10.3390/bios12020069

来源: DOAJ

【 摘 要 】

The differentiation of cultivars is carried out by means of morphological descriptors, in addition to molecular markers. In this work, near-infrared spectroscopy (NIR) and chemometric techniques were used to develop classification models for two different commercial sesame cultivars (Sesamum indicum) and 3 different strains. The diffuse reflectance spectra were recorded in the region of 700 to 2500 nm. Based on the application of chemometric techniques: principal component analysis—PCA, hierarchical cluster analysis—HCA, k-nearest neighbor—KNN and the flexible independent modeling of class analogy—SIMCA, from the infrared spectra in the near region, it was possible to perform the genotyping of two sesame cultivars (BRS Seda and BRS Anahí), and to classify these cultivars with 3 different sesame strains, obtaining 100% accurate results. Due to the good results obtained with the implemented models, the potential of the methods for a possible realization of forensic, fast and non-destructive authentication, in intact sesame seeds was evident.

【 授权许可】

Unknown