Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae

Crop Journal

Yulin Jia¹  Yuntao Dai¹  Eugenia Winston²  James C. Correll³ 

[1] Department of Plant Pathology, University of Arkansas, Fayetteville, AR 72701, USA;USDA Agricultural Research Service, Dale Bumpers National Rice Research Center, 2890 Hwy 130E, Stuttgart, AR 72160, USA;USDA Agriculture Research Service, Jamie Whitten Delta States Research Center, Stoneville, MS 38776, USA;

关键词: Avirulence gene;    AVR-Pita1;    Pi-ta;    Rice blast resistance;   

DOI  :  doi:10.1016/j.cj.2013.12.002

来源: DOAJ

【 摘 要 】

The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature.

【 授权许可】

Unknown