Crop Journal | |
Induction of avirulence by AVR-Pita1 in virulent U.S. field isolates of Magnaporthe oryzae | |
Yulin Jia1  Yuntao Dai1  Eugenia Winston2  James C. Correll3  | |
[1] Department of Plant Pathology, University of Arkansas, Fayetteville, AR 72701, USA;USDA Agricultural Research Service, Dale Bumpers National Rice Research Center, 2890 Hwy 130E, Stuttgart, AR 72160, USA;USDA Agriculture Research Service, Jamie Whitten Delta States Research Center, Stoneville, MS 38776, USA; | |
关键词: Avirulence gene; AVR-Pita1; Pi-ta; Rice blast resistance; | |
DOI : doi:10.1016/j.cj.2013.12.002 | |
来源: DOAJ |
【 摘 要 】
The AVR-Pita1 gene, from the Chinese isolate O-137 of Magnaporthe oryzae, is an effector that determines the efficacy of the Pi-ta rice blast resistance gene. In the present study, the avirulence function of AVR-Pita1 was induced by transformation of field isolates (TM2, ZN19, B2 and B8) that originally were collected from the U.S. and are virulent on Pi-ta-carrying rice cultivars. The presence of AVR-Pita1 from O-137 in independent transformants was detected by PCR using AVR-Pita1 specific primers and verified by DNA sequencing and Southern blot analysis using the AVR-Pita1 coding region as a probe. The results of pathogenicity assays showed that the AVR-Pita1-transformed isolates were not able to infect rice cultivars Katy and Drew carrying Pi-ta. Control isolates that were transformed with inserts lacking the AVR-Pita1 gene remained virulent. Our findings demonstrate that AVR-Pita1 can be used to induce novel gene-specific blast resistance in nature.
【 授权许可】
Unknown