Protective effect of bone marrow mesenchymal stem cells modified with klotho on renal ischemia-reperfusion injury

Renal Failure

Xi Chen¹  Bo Chen²  Li-Bo Xie³  Rui Jiang³  Yi-Ping Lu⁴  Xian-Ding Wang⁴ 

[1] East China Normal University;Southwest Medical University;The Affiliated Hospital of Southwest Medical University;The Institution of Urology, West China Hospital, Sichuan University;

关键词: renal ischemia-reperfusion injury;    bone marrow mesenchymal stem cells;    gene transfection;    klotho;    foxo1;   

DOI  :  10.1080/0886022X.2019.1588131

来源: DOAJ

【 摘 要 】

Objective: To detect the combination protective effect of bone marrow mesenchymal stem cells (BMSCs) and Klotho gene on the renal ischemia-reperfusion injury (RIRI). Methods: BMSCs isolated from rats were transfected with Klotho gene to form BMSCKl. We injected BMSCKl to allogenic rat RIRI model. After 24 h and 72 h, we detected the serum creatinine (SCr), malondialdehyde (MDA), and superoxide dismutase (SOD) in renal tissue, Hematoxylin-eosin (HE) staining, and TUNEL of renal pathology. The expression of FoxO1 and p-FoxO1 in post-hypoxia tubular epithelial cells of normal rat kidney (NRK-52E) were detected by Western blot after cocultured with BMSCKl. Results: Comparing with BMSCCon group, Rats in BMSCKl group had lower SCr and MDA but higher SOD. Both HE and TUNEL score of renal tissue in BMSCKl group were lower than that of BMSCCon group. Western blot indicated that FoxO1 was upregulated, while p-FoxO1 was downregulated in post-hypoxia NRK-52E cells. Conclusions: BMSCs transfected with Klotho gene can further ameliorate RIRI. The possible mechanism may be attributed to the upregulation of SOD in NRK-52E caused by Klotho-FoxO1 axis.

【 授权许可】

Unknown