The Practicability of the Xpert HCV Viral Load Fingerstick Point-of-Care Assay in Primary Care Settings

Viruses

David Petroff¹  Johannes Wiegand²  Thomas Berg²  Katrin Jedrysiak³  Olaf Bätz³  Jan Kramer³ 

[1] Clinical Trial Centre, University of Leipzig, 04107 Leipzig, Germany;Division of Hepatology, Department of Medicine II, Leipzig University Medical Center, 04103 Leipzig, Germany;LADR Laboratory Group Dr. Kramer & Colleagues, 21502 Geesthacht, Germany;

关键词: hepatitis C;    screening;    elimination;    World Health Organization;    check-up;   

DOI  :  10.3390/v13112327

来源: DOAJ

【 摘 要 】

Linkage to care presents one obstacle toward eliminating HCV, and the current two-step pathway (anti-HCV, followed by HCV-RNA testing) results in the loss of patients. HCV screening was tested in the primary care setting with the fingerstick Xpert HCV viral load point-of-care assay to analyze the practicability of immediate diagnosis. Anti-HCV (Cobas) and HCV-RNA (Cobas Amplicor version 2.0, only performed if anti-HCV was positive) were analyzed centrally as the gold standard. The Xpert assay was performed by 10 primary care private practices. In total, 622 patients were recruited. Five individuals (0.8%) were anti-HCV positive, and one was HCV-RNA positive. The Xpert test was valid in 546/622 (87.8%) patients. It was negative in 544 and positive in 2 cases, both of whom were anti-HCV negative. The HCV-RNA PCR and the Xpert test were both negative in 4/5 anti-HCV-positive cases, and the individual with HCV-RNA 4.5 × 10⁶ IU/mL was not detected by the Xpert test. Primary care physicians rated the Xpert test practicability as bad, satisfactory, or good in 6%, 13%, and 81%, respectively, though 14/29 (48%) bad test ratings were assigned by a single practice. Despite adequate acceptance, interpretability and diagnostic performance in primary care settings should be further evaluated before its use in HCV screening can be recommended.

【 授权许可】

Unknown