Identification of QTLs for Reduced Susceptibility to Rose Rosette Disease in Diploid Roses

Pathogens

Mark T. Windham¹  Sara Collins¹  Zena Rawandoozi²  Jeekin Lau²  Oscar Riera-Lizarazu²  David H. Byrne²  Patricia E. Klein²  Ellen L. Young²  Nolan B. Bentley³ 

[1] Department of Entomology and Plant Pathology, Institute of Agriculture, University of Tennessee, Knoxville, TN 37996, USA;Department of Horticultural Sciences, Texas A&M University, College Station, TX 77843, USA;Department of Integrative Biology, University of Texas at Austin, Austin, TX 78705, USA;

关键词: Rosa;    emaravirus;    QTL;    virus resistance;    plant breeding;   

DOI  :  10.3390/pathogens11060660

来源: DOAJ

【 摘 要 】

Resistance to rose rosette disease (RRD), a fatal disease of roses (Rosa spp.), is a high priority for rose breeding. As RRD resistance is time-consuming to phenotype, the identification of genetic markers for resistance could expedite breeding efforts. However, little is known about the genetics of RRD resistance. Therefore, we performed a quantitative trait locus (QTL) analysis on a set of inter-related diploid rose populations phenotyped for RRD resistance and identified four QTLs. Two QTLs were found in multiple years. The most consistent QTL is qRRV_TX2WSE_ch5, which explains approximately 20% and 40% of the phenotypic variation in virus quantity and severity of RRD symptoms, respectively. The second, a QTL on chromosome 1, qRRD_TX2WSE_ch1, accounts for approximately 16% of the phenotypic variation for severity. Finally, a third QTL on chromosome 3 was identified only in the multiyear analysis, and a fourth on chromosome 6 was identified in data from one year only. In addition, haplotypes associated with significant changes in virus quantity and severity were identified for qRRV_TX2WSE_ch5 and qRRD_TX2WSE_ch1. This research represents the first report of genetic determinants of resistance to RRD. In addition, marker trait associations discovered here will enable better parental selection when breeding for RRD resistance and pave the way for marker-assisted selection for RRD resistance.

【 授权许可】

Unknown