Analysis of Transcriptional Changes in Different Brassica napus Synthetic Allopolyploids

Genes

Rui Zhang¹  Shujiang Zhang²  Hui Zhang²  Fei Li²  Yunxiao Wei²  Guoliang Li²  Rifei Sun²  Shifan Zhang² 

[1] Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Beijing 100081, China;

关键词: Brassica napus;    allopolyploid;    transcriptome;    expression level dominance;    trait separation;   

DOI  :  10.3390/genes12010082

来源: DOAJ

【 摘 要 】

Allopolyploidy is an evolutionary and mechanistically intriguing process involving the reconciliation of two or more sets of diverged genomes and regulatory interactions, resulting in new phenotypes. In this study, we explored the gene expression patterns of eight F2 synthetic Brassica napus using RNA sequencing. We found that B. napus allopolyploid formation was accompanied by extensive changes in gene expression. A comparison between F2 and the parent shows a certain proportion of differentially expressed genes (DEG) and activation\silent gene, and the two genomes (female parent (AA)\male parent (CC) genomes) showed significant differences in response to whole-genome duplication (WGD); non-additively expressed genes represented a small portion, while Gene Ontology (GO) enrichment analysis showed that it played an important role in responding to WGD. Besides, genome-wide expression level dominance (ELD) was biased toward the AA genome, and the parental expression pattern of most genes showed a high degree of conservation. Moreover, gene expression showed differences among eight individuals and was consistent with the results of a cluster analysis of traits. Furthermore, the differential expression of waxy synthetic pathways and flowering pathway genes could explain the performance of traits. Collectively, gene expression of the newly formed allopolyploid changed dramatically, and this was different among the selfing offspring, which could be a prominent cause of the trait separation. Our data provide novel insights into the relationship between the expression of differentially expressed genes and trait segregation and provide clues into the evolution of allopolyploids.

【 授权许可】

Unknown