Complete genome sequence of esterase-producing bacterium Croceicoccus marinus E4A9T

Standards in Genomic Sciences

Lin Xu¹  Ying-Yi Huo¹  Yue-Hong Wu¹  Chun-Sheng Wang¹  Hong Cheng¹  Qian Liu¹  Xue-Wei Xu¹ 

[1] Key Laboratory of Marine Ecosystem and Biogeochemistry, Second Institute of Oceanography, State Oceanic Administration;

关键词: Croceicoccus marinus E4A9T;    Genome sequence;    Esterase;    Alphaproteobacteria;   

DOI  :  10.1186/s40793-017-0300-0

来源: DOAJ

【 摘 要 】

Abstract Croceicoccus marinus E4A9Twas isolated from deep-sea sediment collected from the East Pacific polymetallic nodule area. The strain is able to produce esterase, which is widely used in the food, perfume, cosmetic, chemical, agricultural and pharmaceutical industries. Here we describe the characteristics of strain E4A9, including the genome sequence and annotation, presence of esterases, and metabolic pathways of the organism. The genome of strain E4A9T comprises 4,109,188 bp, with one chromosome (3,001,363 bp) and two large circular plasmids (761,621 bp and 346,204 bp, respectively). Complete genome contains 3653 coding sequences, 48 tRNAs, two operons of 16S–23S-5S rRNA gene and three ncRNAs. Strain E4A9T encodes 10 genes related to esterase, and three of the esterases (E3, E6 and E10) was successfully cloned and expressed in Escherichia coli Rosetta in a soluble form, revealing its potential application in biotechnological industry. Moreover, the genome provides clues of metabolic pathways of strain E4A9T, reflecting its adaptations to the ambient environment. The genome sequence of C. marinus E4A9T now provides the fundamental information for future studies.

【 授权许可】

Unknown