Evaluation of pathology and environmental variables contributing to hepatopancreatic necrosis syndrome of Chinese mitten crab, Eriocheir sinensis

Ecotoxicology and Environmental Safety

Huimin Duan¹  Ping Ouyang²  Yang Feng²  Chao Yang²  Shiyong Yang³  Yi Geng⁴  Xiaoli Huang⁴  Lu Zhao⁴  Lizi Yin⁵  Defang Chen⁵ 

[1] Corresponding authors.;Technology, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang 611130, Sichuan, China;;Department of Aquaculture, College of Animal Science &Sichuan Water Conservancy Vocational and Technical College, Chengdu 611231, Sichuan, China;

关键词: Eriocheir sinensis;    Hepatopancreatic necrosis syndrome;    Pathological evaluation;    Multiple correspondence analysis;    Environmental variables;    Animal model;   

DOI  :  

来源: DOAJ

【 摘 要 】

Hepatopancreatic Necrosis Syndrome (HPNS) severely impacts the Chinese mitten crab (Eriocheir sinensis) industry. However, little knowledge of the aetiology and pathogenesis of the disease causes significant difficulties in its prevention and control. In this study, we conducted a pathological analysis of HPNS through time-integrated large-volume sampling, to clarify the disease characteristics and mechanism of HPNS-afflicted crabs; besides, animal models were constructed to verify the pathological diagnosis. The results showed that the hepatopancreas was the principal target organ of HPNS; multiple correspondence analysis revealed that the main histopathological characteristics included non-interstitial atrophic hepatopathy diseases such as hepatic tubule atrophy, dilated hepatic tubules, and hepatic tubule necrosis. Additionally, the muscles also showed signs of disease, including myofibre atrophy, necrosis, and inflammation. Ultrastructural studies showed prominent apoptosis and autophagy-like alterations in the hepatopancreas of HPNS-afflicted crabs. Further, the establishment of animal models revealed that the double variate stimulation of environmental variables such as abamectin/sewage with nutrition deficiency could result in HPNS-similar lesions. Based on these studies, we concluded that HPNS is a chronic hepatopancreas-initiated energy-consumed disease with a low likelihood of pathogen but a high probability of environment and nutrition.

【 授权许可】

Unknown