Development of Plastid Genomic Resources for Discrimination and Classification of Epimedium wushanense (Berberidaceae)

International Journal of Molecular Sciences

Li Ren¹  Baolin Guo¹  Baosheng Liao¹  Xiaohui Pang¹  Jingyuan Song²  Ying Li³  Shilin Chen⁴  Mengyue Guo⁴  Yanqin Xu⁴  Nitin Mantri⁴ 

[1] Peking Union Medical College, Beijing 100193, China;College of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang 330004, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;;Key Lab of Chinese Medicine Resources Conservation, State Administration of Traditional Chinese Medicine of the People’s Republic of China, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences &

关键词: plastid genome;    Epimedium;    phylogenetic analysis;    identification;   

DOI  :  10.3390/ijms20164003

来源: DOAJ

【 摘 要 】

Epimedium wushanense (Berberidaceae) is recorded as the source plant of Epimedii Wushanensis Folium in the Chinese Pharmacopoeia. However, controversies exist on the classification of E. wushanense and its closely related species, namely, E. pseudowushanense, E. chlorandrum, E. mikinorii, E. ilicifolium, and E. borealiguizhouense. These species are often confused with one another because of their highly similar morphological characteristics. This confusion leads to misuse in the medicinal market threatening efficiency and safety. Here, we studied the plastid genomes of these Epimedium species. Results show that the plastid genomes of E. wushanense and its relative species are typical circular tetramerous structure, with lengths of 156,855−158,251 bp. A total of 112 genes were identified from the Epimedium plastid genomes, including 78 protein-coding, 30 tRNA, and 4 rRNA genes. A loss of rpl32 gene in E. chlorandrum was found for the first time in this study. The phylogenetic trees constructed indicated that E. wushanense can be distinguished from its closely related species. E. wushanense shows a closer relationship to species in ser. Dolichocerae. In conclusion, the use of plastid genomes contributes useful genetic information for identifying medicinally important species E. wushanense and provides new evidence for understanding phylogenetic relationships within the Epimedium genus.

【 授权许可】

Unknown