Characterization of Oilseed Lipids from “DHA-Producing Camelina sativa”: A New Transformed Land PlantContaining Long-Chain Omega-3 Oils

Nutrients

Peter D. Nichols¹  Maged P. Mansour¹  Srinivas Belide²  Pushkar Shrestha²  James R. Petrie²  Surinder P. Singh² 

[1] CSIRO Food Futures Flagship, Marine and Atmospheric Research, Castray Esplanade Hobart, Tasmania 7000, Australia;CSIRO Food Futures Flagship, Plant Industry, P.O. Box 1600, Canberra ACT 2601, Australia;

关键词: DHA;    docosahexaenoic acid;    Camelina sativa;    omega-3;    long-chain PUFA;    triacylglycerol;    polar lipids;    glycolipids;    phospholipids;    fish oils;   

DOI  :  10.3390/nu6020776

来源: DOAJ

【 摘 要 】

New and sustainable sources of long-chain (LC, ≥C20) omega-3 oils containing DHA (docosahexaenoic acid, 22:6ω3) are required to meet increasing demands. The lipid content of the oilseed of a novel transgenic, DHA-producing land plant, Camelina sativa, containing microalgal genes able to produce LC omega-3 oils, contained 36% lipid by weight with triacylglycerols (TAG) as the major lipid class in hexane extracts (96% of total lipid). Subsequent chloroform-methanol (CM) extraction recovered further lipid (~50% polar lipid, comprising glycolipids and phospholipids) and residual TAG. The main phospholipid species were phosphatidyl choline and phosphatidyl ethanolamine. The % DHA was: 6.8% (of total fatty acids) in the TAG-rich hexane extract and 4.2% in the polar lipid-rich CM extract. The relative level of ALA (α-linolenic acid, 18:3ω3) inDHA-camelina seed was higher than the control. Major sterols in both DHA- and control camelina seeds were: sitosterol, campesterol, cholesterol, brassicasterol and isofucosterol. C16–C22 fatty alcohols, including iso-branched and odd-chain alcohols were present, including high levels of iso-17:0, 17:0 and 19:0. Other alcohols present were: 16:0,iso-18:0, 18:0 and 18:1 and the proportions varied between the hexane and CM extracts. These iso-branched odd-chain fatty alcohols, to our knowledge, have not been previously reported. These components may be derived from wax esters, or free fatty alcohols.

【 授权许可】

Unknown