Comparison of HE and Giemsa staining in diagnosis of bacterial and allergic conjunctivitis in children

Guoji Yanke Zazhi

Lei Hou¹  Na Gao²  Xue-Lian Hao²  Ze-Feng Kang³ 

[1] Department of Ophthalmology, First People's Hospital of Xining City, Xining 810000, Qinghai Province, China;Department of Ophthalmology, Second People's Hospital of Xining City, Xining 810000, Qinghai Province, China;Eye Hospital of China Academy of Chinese Medical Sciences, Beijing 100040, China;

关键词: hematoxylin-eosin staining;    giemsa staining;    bacterial conjunctivitis;    allergic conjunctivitis;    differential diagnosis;   

DOI  :  10.3980/j.issn.1672-5123.2020.12.34

来源: DOAJ

【 摘 要 】

AIM:To explore the diagnostic effect of hematoxylin-eosin staining(HE)and Giemsa staining in the diagnosis of bacterial and allergic conjunctivitis in children.

METHODS:Totally 422 children with conjunctivitis diagnosed by conjunctivitis from the ophthalmology department of our hospital during 2016-10/2019-10 as the research objects. HE and Giemsa staining methods were used to stain the conjunctival scratches, and the staining results were used to diagnose bacterial/allergic conjunctivitis. Observe the positive detection rate of the two staining results for bacterial/allergic conjunctivitis and the staining situation.

RESULTS: The positive rate(33.0%)and coincidence rate(63.6%)of HE staining for the diagnosis of bacterial conjunctivitis were significantly lower than Giemsa staining(90.7% and 88.8%, P<0.001), while the positive rate of allergic conjunctivitis was not significantly different 90.8% vs 87.2%, P>0.05).

CONCLUSION: The Giemsa staining method can accurately diagnose bacterial conjunctivitis in children and the method is simple. Both HE and Giemsa staining methods have good diagnostic effects on allergic conjunctivitis, which can provide a basis for improving the clinical diagnosis efficiency and early treatment options.

【 授权许可】

Unknown