Seminal Plasma Protein N-Glycan Peaks Are Potential Predictors of Semen Pathology and Sperm Chromatin Maturity in Men

Life

Marcello Ceppi¹  Marco Bruzzone¹  Evangelini Evgeni²  Ana Matijevic³  Tea Petrovic⁴  Gordan Lauc⁴  Iwona Wójcik⁴  Irena Trbojevic-Akmacic⁴  Davor Jezek⁵  Tihana Maric⁵  Ana Katusic Bojanac⁵  Aleksandra Fucic⁵ 

[1] Clinical Epidemiology Unit, IRCCS Ospedale Policlinico San Martino, 16132 Genova, Italy;Cryogonia Cryopreservation Bank, 11526 Athens, Greece;Department of Laboratory Diagnostics, University Hospital Zagreb, 10000 Zagreb, Croatia;Genos Glycoscience Research Laboratory, 10000 Zagreb, Croatia;Scientific Centre of Excellence for Reproductive and Regenerative Medicine, University of Zagreb School of Medicine, 10000 Zagreb, Croatia;

关键词: male infertility;    sperm;    seminal plasma;    N-glycosylation;    DNA fragmentation;    Halosperm;   

DOI  :  10.3390/life11090989

来源: DOAJ

【 摘 要 】

Background: Male infertility is increasingly becoming a health and demographic problem. While it may originate from congenital or acquired diseases, it can also result from environmental exposure. Hence, the complexity of involved molecular mechanisms often requires a multiparametric approach. This study aimed to associate semen parameters with sperm DNA fragmentation, chromatin maturity and seminal plasma protein N-glycosylation. Methods: The study was conducted with 166 participants, 20–55 y old, 82 normozoospermic and 84 with pathological diagnosis. Sperm was analyzed by Halosperm assay and aniline blue staining, while seminal plasma total protein N-glycans were analyzed by ultra-high-performance liquid chromatography. Results: Sperm DNA fragmentation was significantly increased in the pathological group and was inversely correlated with sperm motility and viability. Seminal plasma total protein N-glycans were chromatographically separated in 37 individual peaks. The pattern of seminal plasma N-glycan peaks (SPGP) showed that SPGP14 significantly differs between men with normal and pathological semen parameters (p < 0.001). The multivariate analysis showed that when sperm chromatin maturity increases by 10%, SPGP17 decreases by 14% while SPGP25 increases by 25%. Conclusion: DNA integrity and seminal plasma N-glycans are associated with pathological sperm parameters. Specific N-glycans are also associated with sperm chromatin maturity and have a potential in future fertility research and clinical diagnostics.

【 授权许可】

Unknown